Human nasal microvascular endothelial cells were isolated and cultured from the inferior turbinate. Endothelial cells grew rapidly to confluence and survived serial passages until the eighth subculture in a medium composed of MCDB 107, 10% fetal bovine serum, 75μg/ml endothelial cell growth supplement and 24units/ml insulin in the gelatin-coated 60mm dish. The cells were identified as endothelial cells by their morphology and immunofluoresence stain for human factor VIII antigen. Capillary-like tubule formation was usually noted 4-6weeks after confluence. We succeeded in long-term culture of human nasal microvascular endothelial cells from the inferior turbinate. This will provide good model for studying the role of endothelial cells.