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Korean Journal of Otorhinolaryngology-Head and Neck Surgery > Volume 33(4); 1990 > Article
Korean Journal of Otorhinolaryngology-Head and Neck Surgery 1990;33(4): 731-40.
Classification of the Carbohydrate in the Rat Soft Palate Using Lectin Binding Method
In Kyu Yoon, MD1, Hyung Duk Oh, MD1, Sang Hwa Lee, MD1, Jin Jeong Kim, MD2, and Jae Bong Kim, MD2
1;Department of Otolaryngology, St. Benedict Hospital, Pusan, 2;Department of Anatomy, College of Medicine, Pusan National University, Korea
성체 흰쥐의 연구개에 있어서 Lectin 결합능을 이용한 탄수화물의 종류
윤인규1 · 오형덕1 · 이상화1 · 김진정2 · 김재봉2
부산 성분도병원 이비인후과1;부산대학교 의과대학 해부학교실2;
ABSTRACT

This study was performed in order to identify the glycoproteins containing oligosaccharides in adult rat soft palate. After making serial paraffin sections at 4μm, the specimens were incubated with 9 species of lectins(UEA-I, BS-I, DBA, SBA, WGA, PNA, PNA after neuraminidase treated, Con-A, RCA-I, LCA). In order to increase specificity of reactions, the sections were applicated with ABC system. And then the sections were incubated DAB and were counterstained with hematoxylin. The other sections were also stained with hematoxin and eosin. The results summarized were as follow ; 1) In the epithelium : a) The coats of cell membranes in the granular cell layer were stained with SBA. b) The coats of cell membranes in the upper spinous cell layer were stained with UEA-I, SBA, WGA, Con-A and RCA-I. c) The coats of cell membranes in the lower spinous cell layer were stained with UEA-I, BS-I, SBA, WGA, PNA, PNA after neuraminidase treated, Con-A and RCA-I. d) The coats of cell membranes in the basal cell layer were stained with BS-I, WGA, PNA after neuraminidase treated Con-A, RCA-I and LCA. 2) In the basement membrane, all lectins used were not stained. 3) In the lamina propria, the connective tissue matrix was stained with UEA-I, BS-I, DBA, WGA, PNA after neuraminidase, Con-A, RCA-I and LCA. 4) In the mucous gland, the mucous cell were stained with UEA-I, DBA, WGA, RCA-I and LCA.

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