Basophils and mast cells are well-known effector cells in nasal allergy. The numbers of basophilic cells in nasal mucosa and nasal secretion are incerased in perennial allergic rhinitis. The mechanism of the migration of human basophils from blood stream to nasal mucosa and nasal secretion has not been clarified. At the present time, it is not clear whether there are different factors in peripheral blood or whether the differences are caused by generating and testing methods. In vitro studies were serially performed to make it clear the chemotactic response of human basophils to cytokine derived chemotactic factors. The peripheral blood basophils were taken from untreated patients of perennial allergic rhinitis and chemotactic assay was performed. There was no significant differences in migrated basophils to supernatants from lymphocytes incubated with PHA of allergic rhinitis patients and non-allergic subjects. Therefore basophils of allergic rhinitis patient had no significant different reactions to supernatants from lymphocytes incubated with PHA between allergic and non-allergic subjects. This suggests that a large number of different stimuli can induce basophils to secrete a variety of mediators. It seems that any other factors activate different biochemical pathways in the basophil chemotaxis.