Microresidual or micrometastasis is one of the most difficult problem in the management of cancer even though there has been a remarkable progress in this field. One of the treatment used for it was chemotherapy, but its result leaves much to be desired. Recently many investigators developed immunological method to solve this hard problem. One of the most well known and effective treatment is adoptive immunotherapy. It is to reinfuse patients' cytotoxic lymphocyte induced by various sensitization procedures. In this study, the generation of cytotoxic cells which was induced by mixed lymphocyte tumor cell culture and propagated with interleukin-2 was done to investigate the possibility of adoptive immunotherapy of head and neck cancer. We observed cytotoxicity of cytotoxic cell against autologous and allogenic tumor cell. The findings obtained were : 1) Cytotoxic cell number was increased about 30 fold in 12 days. 2) Cytotoxic cell induced by mixed lymphocyte tumor cell culture has more cytotoxicity against autologous and allogenic tumor cell than that of PBL. 3) Adoptive immunotherapy to reinfuse cytotoxic lymphocyte may become one of the effective method to treat micrometastasis and microresidual tumor of the head and neck.