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Korean Journal of Otorhinolaryngology-Head and Neck Surgery 1988;31(6): 893-902. |
Immunocytochemical Study of Somatostatinergic Neurons in the Vestibular Ganglion of the Rabbit(Oryctolagus Cuniculus Domesticus) - I. Light Microscopical Structure - |
Sung Ho Choi, MD, Do Gi Kim, MD, Yoon Young Chung, MD, Hyuck Soon Chang, MD, and Ju Won Kang, MD |
Department of Otolaryngology, College of Medicine, Soonchunhyang University, Korea |
집토끼 전정신경절의 Somatostatin신경절세포에 대한 면역세포화학적 연구 - I. 광학현미경적 관찰 - |
최성호 · 김도기 · 정윤영 · 장혁순 · 강주원 |
순천향대학교 의학부 이비인후과학교실 |
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ABSTRACT |
This study was attempted to investigate the distribution, shape and light microscopical characteristics of somatostatin immunoreactive ganglion cells in the vestibular(Scarpa's) ganglion of the rabbit(Oryctolagus cuniculus domesticus). For the light-microscopical examination of immunocytochemistry, the Scarpa's ganglia were fixed in glutaraldehyde-picric acid-acetic acid(GPA) or 10% neutral buffered formalin solutions by means of perfusion. For identification of the somatostatin immunoreactive ganglion cells, the paraffin sections(6 μm) were stained immunocytochemically by PAP procedure(Sternberger, 1979) with rabbit anti-human synthetic somatostatin(Dakopatts, C.A., U.S.A.). The other side, we stained the sections with toluidine blue or HCl-lead hematoxylin for the microscopical characteristics of somatostatinergic neurons before PAP stain. And with the light microscope, we identified the frequency, shape and characteristics of somatostatin immunoreactive ganglion cells. Vestibular ganglion cells of the rabbit were classified into three types ; large non-immunoreactive ganglion cells(25μm × 17μm), small non-immunoreactive ganglion cells(15μm × 11μm) and immunoreactive ganglion cells(28μm × 22μm), and each frequency were 71.7%, 6.4% and 21.9%. All somatostatin immunoreactive ganglion cells were large neurons(28μm × 22μm) and scattered in the Scarpa's ganglion. These ganglion cells were oval or spherical in shape and contained well developed Nissl's bodies which were distributed in the cytoplasm.
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